Laboratory safety:
Wear appropriate personal protective equipment, such as lab coats, gloves, and safety glasses.
The experimental area should be kept clean to avoid contamination.
When handling samples, avoid direct contact and use a biosafety cabinet or appropriate protective equipment.
Sample handling:
Samples should be properly labeled to avoid confusion.
Samples should be sterilized before handling to ensure aseptic operation.
Strictly follow the steps during DNA extraction to avoid DNA degradation or contamination.
Reaction mixture preparation:
Use dedicated PCR reagents and strictly follow the formula.
Pay attention to the preservation of reagents and avoid long-term exposure to light.
Avoid excessive or insufficient reaction components to ensure the accuracy of the reaction.
PCR program settings:
Set appropriate cycle conditions according to experimental requirements to avoid DNA damage caused by overheating and overheating.
Use a stable PCR instrument to ensure the accuracy of temperature control.
PCR reaction process:
Strictly follow the procedure to avoid interruption or premature termination.
Keep the reaction wells clean to avoid cross contamination.
Product analysis:
Use appropriate electrophoresis conditions to ensure clear product separation.
Fluorescence scanning data should be analyzed by professional software to avoid human errors.
Data processing and reporting:
Data should be accurately recorded, including original images and analysis results.
Repeatedly verify the results to ensure reliability.
Waste disposal:
Properly dispose of PCR waste, including samples, PCR reaction solutions and waste, according to local regulations.
Regular calibration and maintenance:
PCR instruments should be calibrated and maintained regularly to ensure stable performance.