Vitamin D Rapid Test

PocTell^® Vitamin D Rapid Test Device (Whole Blood/Serum/Plasma) is used for semi-quantitative determination and monitoring of 25-OH Vitamin D concentrations in whole blood/serum/plasma specimens..

PocTell^® Vitamin D Rapid test sample which is diluted with buffer solution add to the sample well of the test device. The sample now moves through the test strip from bottom to top. If the test sample contains 25-OH Vitamin D, it attaches to the first anti-25-OH Vitamin D antibody which is conjugated with a red gold colloidal for color marking. The red 25-OH Vitamin D antibody-gold complex, together with the sample liquid, diffuses through the membrane that is pre dispensed with lines of different amounts of the second anti-25-OH Vitamin D antibody. The 25-OH Vitamin D-antibody-gold complex is immobilized by the antibodies coated on the membrane leading to the formation of red lines. The number of lines depends on the 25-OH Vitamin D concentration in the sample.

The control line serves as a procedural control and indicates that sufficient volume of specimen has been added and membrane wicking has occurred. 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1.Bring the pouch to room temperature before opening it. Remove the test device from the sealed pouch and use it as soon as possible.

2.Place the test device on a clean and level surface.

For Serum, Plasma or Venipuncture Whole Blood specimens:

Hold the dropper vertically and transfer 1 drop of specimen (approximately 25 L) into the Specimens dilution tube with buffer(approximately 200 L), mixed well , then add 3 drops (approximately 80ul-120ul) of mixed solution into the sample well and start the timer.

For Fingerstick Whole Blood specimens:

To use a capillary tube: Allow Fill the capillary tube and transfer approximately 25 L of fingerstick whole blood specimen into the Specimens dilution tube with buffer(approximately 200 L), mixed well , then add 3 drops (approximately 80ul-120ul) of mixed solution into the sample well and start the timer.

To use a dropper::Hold the dropper vertically and transfer 1 drop of specimen (approximately 25 L) into the Specimens dilution tube with buffer(approximately 200 L), mixed well , then add 3drops (approximately 80ul-120ul) of mixed solution into the sample well and start the timer.

3.Wait for the colored line(s) to appear. Read the result at 10-15 minutes. Do not interpret the result after 20 minutes. .

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